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A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All Test
Test Sample Vol Storage Cut off time Reporting
times
Special
instructions
Absolute Eosinophil
Count (AEC) 
WB  200mL  R 3pm  SD   
Adenosine Deaminase
(ADA) 
S / Fluid 2 mL R 12pm  ND   
AFB/ZN stain Fluid/Pus/
Smears
      SD   
Albumin  S / PL 200 mL  R 3pm  SD    
Alk  Phosphatase  S / PL 200 mL  R 3pm  SD    
Alpha Feto Protein
(AFP) 
S / Fluid 2 mL R 12pm  SD   
AMA  By  Fluiduorescence
(Anti mitochondria
antibody) 
2mL  R 12pm W, S F,T  
Ammonia  P 2 mL R 12pm  SD   
Amylase (Alpha)  S/P/ Fluid 2 mL R 3pm  SD    
ANA  By 
Fluiduorescence
2 mL  R   Mo–Th   
ANCA  P & C, with titre 2 mL R M,W,F; 3 PM W,F,M  
ANCA  P & C, without
titre
2 mL R M,W,F; 12 PM W,F,M  
Anti  Cardiolipin  (ACL)
Ig M 
2 mL R 12pm  SD   
Anti  Cardiolipin 
(ACL)  Ig G 
2 mL R 12pm  SD   
Anti  ds DNA   2 mL    3pm PD  Mo – Th   
Anti  Tub Ig A  S / Fluid   R 12pm  SD   
Anti  Tub Ig G  S / Fluid 2 mL  R 12pm  SD   
Anti  Tub Ig M   S / Fluid 2 mL  R 12pm  SD   
Anti Thrombin III –
Functional 
200mL    3pm  4th day   
APO Lipoprotein  A1 
(APO A1) 
2 mL R 12pm  SD   
APO Lipoprotein B
(APO B) 
2 mL R 12pm  SD   
ASLO / ASO, latex,
with titre
2 mL  R   SD   
ASMA By 
Fluiduorescence (Anti
smooth muscle
antibody ) 
2mL  R 12pm W, S F,T  
B12 / Vitamin B12  S 2mL  R 12pm  SD   
Bicarbonate (HCO3-)  P / S  200mL  R   SD   
Bilirubin BU/BC
(Unconjugated &
Conjugated Bilirubin ) 
P / S  200mL    3pm  SD   
Bilirubin Total  P / S  200mL      SD   
Biopsy-Appendix - - A - 6th Day  
Biopsy-Bone marrow     A   7th day Detailed history
and WB in
EDTA +
Aspiration
Biopsy-Bone marrow
with procedure
        7th day  
Biopsy-Endometrium - - A - 6th Day  
Biopsy-ENT Punch, GI
endoscopy, skin,
Cervix, Fallopian tube.
(upto 2 containers)
- - A - 6th Day  
Biopsy-Gallbladder,
Uterus, Small lymph
node, Breast lump,
Synovium, Fistula,
Sinus, Fibroid, Hernia
sac, disc lesion
- - A - 6th Day  
Biopsy-Large ovarian
tumors, Wide excision
specimen (without
lymph node dissection),
only lymph node
dissection
(RND/MND/SOHD/
Inguinal node
dissection etc.),
Intestine
- - A - 6th Day  
Biopsy-Prostate any
tissue, bladder tumor,
CT guided biopsy,
Lymph nodes, Thyroid
nodule, Ovarian
cyst/tumor, All needle
biopsies
- - A - 6th Day  
Biopsy-Radical surgery - - A - 6th Day  
Biopsy-Thyroidectomy,
Small soft tissue tumor,
Brain tumor, Bone
biopsy
- - A - 6th Day  
Blood  Group  WB EDTA  200mL    3pm  SD    
Body  Fluids  Pleural /
Ascitic / Synovial /  BAL
/ PLericardial /
Dialysis  
Fluid  5mL    3pm  SD   
Bone marrow aspiration BM   R   2nd day  
Brucella – IgG 2mL  R 12pm T,F Th, M  
Brucella – IgM 2mL  R 12pm T,F Th, M  
Brucella antibodies
by agglutination
2mL  R 12pm SD   
BT Screen  ---  NA    3pm  SD   
Ca – 125  Ovarian
Marker 
2 mL R 12pm  SD   
Ca - 15 3 Breast 
Marker 
S 2 mL R 12pm  SD   
Ca - 19 9 GI  Marker  S 2 mL R 12pm  SD   
Calcium  S / PL / Urine  200mL  R 3pm  SD   
Calcium, Ionized   200mL  R 3pm  SD   
Carbamezapine Drug
Assay 
S 2 mL R 12pm  SD  Mention last
dose of drug.
Collection time   
CD marker for acute
leukemia
WB/BM 6 mL R   ND   
CD marker for
CLL/Lymphoma
WB/BM 6 mL R   ND   
CD-117/c-kit Blocks/Specimen   A   4th Day   
CEA  S /  Fluid  2mL  R 12pm  SD   
Chloride (Cl-)  S / PL / Urine  200mL  R   SD  All four
electrolytes=
400
Cholesterol  S / PL / Fluid  200mL  R   SD    
Cholinesterase  S 2mL  R   2 hrs  
CK  (CPK)  S / PL  2mL  R   SD    
CK-MB  S / PL  200mL  R   SD   
Clot  Retraction Time
(CRT) 
WB  1mL  A 3pm  SD  Contact lab
CMV Blocks/Specimen   A   4th Day   
CMV  Antibody  Ig G   S / CSF 2mL  R 12pm SD   
CMV  Antibody Ig M   S / CSF 2mL  R 12pm SD   
CMV IHC     A   3 Days  
Cold  Agglutinins            Contact lab
Cold And Warm
Antibodies 
          Contact lab
Complement C3  2mL  F 12pm SD   
Complement C4  2mL  F 12pm SD   
Complete Blood Count  WB  2mL  R   SD   
Coomb's Direct WB  2mL  R   SD   
Coomb's Indirect 200mL  R 3pm  ND  Note pregnancy
weeks if any 
Cortisol, blood S 2mL R 12pm  SD  Mention time
of collection
and drug
history
Cortisol, urine 24 hrs urine  10 mL R 12pm  SD   
Creatinine    S / PL / Urine  200mL  R   SD    
CRP S / PL  2 mL R   SD    
Cryoglobulins Screen            Contact lab
CSF Protein  CSF 200mL  R   SD    
CSF Routine  CSF  1mL  R   SD   
Culture  Fungus  Blood /  Pus / Fluid
/ Swab /Sputum 
NA  R 7pm  7th  and
14th day 
 
Culture & Sensitivity
Pyogenic 
Pus / Fluid / Swab  NA  R 8pm  3rd day   
Culture & sensitivity
pyogenic (Bactec)
WB / Fluid / PLus /
swab / urine
NA  R 7pm  5 days   
Cytogenetics studies -
hematological disorder
WB/BM 6 mL R   12th Day  
Cytogenetics studies -
stress cyto
BM 6 mL R   12th Day  
Dengue Antibody IgG +
IgM 
SR / PLL  2mL R   SD   
DHEAS  SR  2mL R 12pm SD   
Digoxin Drug Assay  SR 2mL R 12pm  SD  Note last dose
timing,
Collection time
DNA Extraction for HLA
typing 
WB 2 mL R      
ER Blocks/Specimen   A   4th Day   
ER+PR+Her-2 Blocks/Specimen   A   4th Day   
ESR  WB  2 mL R   SD   
Factor  IX  PL  2 mL R   SD  Contact lab
Factor  VIII  PL  2 mL R   SD  Contact lab
Factor XIII (Screen)  PL  2 mL R   ND  Contact lab
Ferritin  S 2 mL R 12pm  SD   
Fibrinogen  PL  2 mL R   SD   
FISH  BCR-ABL WB/BM 4 mL R 5pm 4th Day   
FISH  LSI BCL 2 /IGH
OR t(14,18)
WB/BM 4 mL R 5pm 4th Day   
FISH 14q32.3 LSI IGH
gene breakapart
WB/BM 4 mL R 5pm 4th Day   
FISH BCL1 WB/BM 4 mL R 5pm 4th Day   
FISH BCL2 OR 18q21 WB/BM 4 mL R 5pm 4th Day   
FISH BCL6/IGH OR
t(3,14)
WB/BM 4 mL R 5pm 4th Day   
FISH BMT SEX
MISMATCH X,Y PROBE 
WB/BM 4 mL R 5pm 4th Day   
FISH BURKITT'S
LYMPHOMA, t(8,14)
WB/BM 4 mL R 5pm 4th Day   
FISH CLL PANEL WB/BM 4 mL R 5pm 4th Day   
FISH del 13q WB/BM 4 mL R 5pm 4th Day   
FISH HER 2/neu
oncogene amplification
Paraffin block   A 5pm 4th Day   
FISH inv 16 or LSI CBFB WB/BM 4 mL R 5pm 4th Day   
FISH LSI BCL1/IGH OR
t(11,14)
WB/BM 4 mL R 5pm 4th Day   
FISH LSI MLL gene
breakapart or 11q23
WB/BM 4 mL R 5pm 4th Day   
FISH MDS PANEL WB/BM 4 mL R 5pm 4th Day   
FISH t (15,17) PML/ RA WB/BM 4 mL R 5pm 4th Day   
FISH t(11,14)
OR LSI IGH /CCNDI
WB/BM 4 mL R 5pm 4th Day   
FISH t(12,21) or  LSI
TEL /AML1
WB/BM 4 mL R 5pm 4th Day   
FISH t(8,21) OR AML1
/ETO
WB/BM 4 mL R 5pm 4th Day   
FLC/Free Light Chain
Assay
WB 2 mL R     contact lab
Flow cytometry for PNH WB 6 mL R   ND   
FLP1 L1 PDGFR WB/BM 2 mL R   contact lab
Fluid for cytology Fluid/Pus 10 mL R - ND   
Fluid for cytology, BAL BAL 10 mL R - ND   
FNAC Doctor's visit - - - - SD  Consent
required
FNAC in house - - - - SD  Consent
required
FNAC, CT guided  - - - - SD   
Folate / Folic  Acid, RBC S + P 2 mL R 3pm  ND   
Folate / Folic  Acid,
serum
S 2 mL R 3pm  ND   
FSH  S 2 mL R 12pm  SD  LMP required
FT3  S 2 mL R 12pm SD   
FT4  S 2 mL R 12pm SD   
Fungal smear /
KOH preparation
Scrapping/Swab/
Aspirates
      SD    
G 6 P D Qualitative  WB  2 mL A   SD   
G 6 P D Quantitative WB  2 mL A 12pm SD   
Gamma  GT  S/ PL 2 mL R   SD    
Glucose    (Sugar)  P / Fluid 2 mL R   SD  FBS - minimum
8 hours fasting.
PP2BS- exactly
2 hours after
meal. PG2BS-
2 hours after
glucose load
RBS-anytime
Gram's  Stain Scrapping/Swab/
Aspirates
      SD    
Growth  Hormone  S 2 mL R 12pm ND  Fasting and
rest for 15 min
Hb  Electrophoresis  WB  2 mL R 3pm PD  Mo – th  Note history of
BT, family
disease 
Hb A 1c (GHB)  WB  2 mL R   SD    
HCG   S/CSF/U 2 mL, Urine 10 mL R 12pm SD  Note LMP and
urine HCG
reports if any 
HCG - Urine qualitative
(preganancy test)
2 mL R   SD  First morning
sample
preferable 
HDL Cholesterol S 2 mL R 12pm SD  Fasting of
minimum 10 hr
Hepatitis A Marker -
Anti  HAV  Ig M 
S 2 mL R 12pm  SD   
Hepatitis A Marker -
Anti  HAV Total/Ig G
S 2 mL R 12pm  SD   
Hepatitis B Marker -
ANTI HB c Ig M (Quantitative) 
S 2 mL R 12pm  SD   
Hepatitis B Marker -
Anti HB c Total/ Ig G 
S 2 mL R 12pm  SD   
Hepatitis B Marker -
ANTI HB e 
S 2 mL R 12pm  SD   
Hepatitis B Marker -
Anti HB s 
S 2 mL R 12pm  SD   
Hepatitis B Marker –
HBeAg 
S 2 mL R 12pm  SD   
Hepatitis B Marker –
HBsAg
S/P/ Fluid 2 mL R   SD    
Hepatitis C Marker -
Anti HCV IgM
antibodies
S 2 mL R 3pm 7th day  
Hepatitis C Marker -
Anti HCV total
antibodies by ELISA
S 2 mL R 3pm ND   
Hepatitis C marker -
Anti HCV, screening
test
S/P 2 mL R   SD   
Hepatitis E Marker -
Anti HEV Ig G
S 2 mL R 3pm S,W Tu,Th  
Hepatitis E Marker -
Anti HEV Ig M 
S 2 mL R 12pm SD   
Her-2/c-erb B2 Blocks/Specimen   A   4th Day   
HIV – Western blot S/CSF 2 mL R 3pm ND   
HIV Screening test S/P/ Fluid 2 mL R   SD    
HLA Typing WB 10 mL R      
Homocystiene, blood S/P 2 mL R 12pm  SD   
Homocystiene, urine U 50 mL R 3pm 4th day   
HSV-I & II, IgG S / CSF  2 mL R 3pm ND   
HSV-I & II, IgM S / CSF  2 mL R 3pm ND   
HSV-I IgG S / CSF  2 mL R 3pm ND   
HSV-I IgM S / CSF  2 mL R 3pm ND   
HSV-II IgG S / CSF  2 mL R 3pm ND   
HSV-II IgM S / CSF  2 mL R 3pm ND   
Ig E  2 mL R 12pm SD   
IHC Blocks/Specimen   A   4th Day   
Immunofixation   S / urine  2 mL   3pm  4th day   
Immunoglobulins  IgA,
blood
2 mL R 3pm ND   
Immunoglobulins  IgA,
CSF
CSF 2 mL R 3pm ND   
Immunoglobulins  IgG,
CSF
CSF 2 mL R 12pm SD   
Immunoglobulins 
IgG,serum
S 2 mL R 12pm SD   
Immunoglobulins 
IgM,CSF
CSF 2 mL R 12pm SD   
Immunoglobulins 
IgM,serum
S 2 mL R 12pm SD   
Insulin  S 2 mL R 12pm  SD  Note fasting or
post prandial
status 
Intraoperative imprint
cytology
- - - - - Contact lab
IRMA   2 mL R     Contact lab
Iron + TIBC S 3 mL R 12pm SD   
JAK 2 Mutation WB/BM 6 mL R      
LAP  Score  WB + five fresh
blood smears
2 mL R   SD   
LDH  S / PL / Fluid  2 mL R   SD    
LDL Cholesterol  S 2 mL R 12pm SD  Fasting of
minimum 10
hr
Lepra Bacilli smear
(slit skin smear)
        SD  contact lab
Leptospira detection P + U 3 mL + spot urine A 3pm  ND   
Leptospira detection
and culture
P + U 3 mL + spot urine R 3pm  1-6
weeks
 
Leptospira-IgG
antibody
S 2 mL R 3pm  ND   
Leptospira-IgM
antibody
S 2 mL R 3pm  ND   
LH  2 mL R 12pm  SD  Mention Age
and LMP
Lipase  S / PL / Fluid  2 mL R   SD    
LIPID Profile S 3 ML R 5pm SD   
Lipoprotein a - Lp(a)  2 mL R 12pm SD  12 h fasting
required 
Lithium Drug Assay  2 mL R 12pm  SD  To collect after
12 hrs of
medication
Liver function test S 3 ML R 5pm SD   
Lupus Anticoagulant
(DRVVT)
2 mL R   SD   
M.P. (Malarial Parasites)  WB  2 mL R   SD   
M.T. (Mantoux, Tuberculin) --  2 mL   --  Between
48 – 72
hrs 
  
Magnesium  S 2 mL R   SD    
Methemoglobin  WB  2 mL A 3pm  ND   
Microalbuminuria 
(MAU) 
U 10 mL R 12pm SD  Spot OR 24 hr
sample without
preservative
Microfilaria  WB  2 mL R   SD  Night 12 – 3
am collection
preferred
Oestradiol (E2)  S 2 mL R 12pm  SD   
Oestriol (E3), blood S 2 mL R 12pm SD   
Oestriol (E3), urine U 24hr 10 mL R 12pm 5th day  
Osmolality            Only calculated
parameter
Osmotic Fragility  / Nestroft            Contact lab
P  Falciparum + P
Vivax Antigen (MP
antigen)
WB  2 mL  R   SD    
Pap smear Smears - A - 4th Day  Age required
PAS stain Smears/WB 2 mL  R   ND    
Paul Bunnel test 2 mL R 12pm  SD   
Phenobarbitone Drug
Assay 
S 2 mL R 12pm  SD  Note time of
last dose 
Phenytoin Drug Assay  S 2 mL R 12pm  SD  Note time of
last dose 
Phosphorus  S 2 mL R   SD    
Platelets  WB  2 mL R   SD   
PNH - Flowcytometry WB 2 mL R 4PM ND   
PNH Screen 
Sucrose Lysis + Acid
Hem Test 
    A     Contact lab
Potassium (K+)  S / PL / urine  2 mL R   SD   
PR Blocks/Specimen   A   4th Day   
ProC Global  2 mL See note 3pm  3rd day  Anti coagulant
therapy – stop
at least 12
days 
Progesterone  S 2 mL  R 12pm  SD  Mention age
and LMP
Prolactin  2 mL  R 12pm  SD  Collect at rest
before 12
noon
Protein 
electrophoresis
S / urine  2 mL  R 3pm PD  We – sa  Fasting sample
is preferred
Protein Total  S / PL / Fluid  2 mL R   SD    
Prothrombin  Time
(PT/PRT)
2 mL R   SD  Mention any
medication
history
PSA  (Free PSA)  S 2 mL R 3pm  ND    
PSA  (Total)  2 mL R 12pm SD    
PTT / APTT  2 mL  R   SD  Mention any
medication
history
RA S 2 mL  R    SD   
RA, quantitative S 2 mL R 12:00 PM SD   
Reticulocyte count WB  2 mL     SD    
Rh  Antibody  Titre  2 mL R   ND  Note blood
group and
pregnancy
weeks 
RPR   S / PL  2 mL   3pm  SD   
Rubella  Antibody  Ig G  S / CSF 2 mL R 12:00 PM SD   
Rubella  Antibody Ig M  S / CSF 2 mL R 12:00 PM SD   
Second opinion with
IHC
    A   5th day  
Second opinion-
cytology/biopsy (No IHC)
    A   3rd day  
Semen analysis SF    A   SD  Contact lab
SGOT (AST)  S / PL  200mL  R 3pm  SD    
SGPT (ALT)  S / PL  200mL  R 3pm  SD    
Sickling test WB  2 mL  R   ND    
Sodium (Na+)  S/P/urine/  2 mL R   SD    
Specific IHC marker
(per marker)
Blocks/Specimen   A   4th Day   
Stool Routine     R   SD   
Sudan Black  Smears/WB 2 mL  R   ND    
T3  2 mL R 12:00 PM SD   
T4  2 mL R 12:00 PM SD   
Testosterone, total S 2 mL R 12pm  SD  mention age
and sex
Thrombophilia mutation study WB 10 mL R      
Thyroglobulin  Ab. /
TGA / Anti
Thyroglobulin /
Antithyroid antibody
(ATA)
S 2 mL R 3pm  SD   
Thyroid Microsomal
(Peroxidase) Ab.[
( TMA / TPO)] 
S 2 mL R 3pm  SD   
TIBC S 2 mL R 12pm  SD   
Toxoplasma  Ab  Ig G  S/CSF 2 mL R 12pm  SD   
Toxoplasma  Ab Ig M  S/CSF 2 mL R 12pm  SD   
TPHA   S / PL  200mL    3pm  SD    
Triglycerides  S / PL  2 mL R   SD  Fasting 12 hrs
preferred
Troponine  I  S / PL  200mL    3pm  SD    
TSH - S (TSH 
Ultrasensitive) 
2 mL R 12pm  SD   
Urea / BUN  S / PL / Fluid  2 mL R   SD    
Uric  Acid  S / PL  /  urine  2 mL R   SD    
Urine  Bence  Jones 
Proteins 
urine  50 mL R   SD  First morning
urine sample
Urine  Hemosiderin  urine  20 mL     SD    
Urine  Routine  urine  20 mL     SD    
Urine Protein  urine        SD  24 h or spot
sample.
Preservative 5
mL 6 mol/L HCL
Widal Slide  Test  2 mL     SD   
 
 
 
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